Quantifying adeno-associated virus (AAV) titer and checking for aggregates are major challenges for developing and manufacturing these gene therapy vectors. The classic AAV tools include chromatography, AUC, TEM, ELISA, and PCR-based methods, but none of these deliver results quickly, easily, and from low sample volumes. When it comes to optimizing processes or running extensive DoEs, faster and easier titers enable evaluation of all the constructs and conditions you want. Since AAV aggregates can mess up the results from many of the classic tools, spotting aggregation before it causes issues can save lots of time and sample.
Stunner gives researchers a full read-out on AAV aggregation and titer on any serotype using only 2 µL of sample. By combining high-speed UV/Vis spectroscopy with static and dynamic light scattering (SLS & DLS), Stunner measures titers down to 1e12 vg/mL and checks size and aggregation of 96 samples in just 1 hour.