Protein quantification & aggregation

The problem

In the early days of working with a new protein, you never knew if you could trust it. Maybe yield isn’t what it used to be or the purification process isn’t quite optimized. Maybe aggregates are hiding out, ready to spoil whatever next assay you’ve got planned. Getting to know the protein sample you’re working with, in terms of how much you have and if it’s aggregated, is the homework that needs to happen to save time and precious sample before extended characterization or assay development studies.

The right tool for the job

Stunner reveals more about your proteins with a combo of high-speed UV/Vis analysis to measure concentration and dynamic light scattering (DLS) to check size and look for aggregates. Stunner uses 2 µL per sample and measures up to 96 samples in just one hour for both UV/Vis and DLS protein analysis. So at the same time concentration is measured, Stunner uses DLS to measure the hydrodynamic diameter of your samples and identify whether aggregates might be a problem. After reading a protein on Stunner, you’ll know exactly what you’re working with.

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The proof

Same-time quant and sizing

Reading UV/Vis and DLS on the same sample shows if there’s any aggregation by hydrodynamic diameter, checks uniformity from polydispersity, and gets the exact concentration all at once. A higher concentration sample may not be the best if it’s also got huge aggregates mixed in.

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Quant it like you want it

Protein quantification by Stunner’s UV/Vis just makes sense - you don’t need any reagents, or standards. It's got a dynamic range that covers from 0.02 mg/mL to 200 mg/mL (mAb), so you can run any protein without dilutions too. Stunner’s two fixed pathlengths deliver spot-on data across its whole concentration range — with accuracy within 2% and precision within 1%.

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See what’s in there

While you’re checking for aggregates, why not check for impurities too? Stunner’s UV/Vis is equipped with Unmix algorithms that can dissect an absorbance spectrum to look for what else might be lurking in there. You’ll see if your A280 is impacted by turbidity, nucleic acids, EDTA or other impurities, and have the power to quantify your protein despite those problems.

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Stunner

Stunner is the only system that pulls together UV/Vis concentration, Dynamic Light Scattering (DLS) and Static Light Scattering (SLS) data from the same 2 μL sample. Dig in to your AAV to get the total capsid titer and empty/full ratio, or rack up payload concentration and size data on any nanoparticle all at once. Nail down your protein quality by knocking concentration, hydrodynamic size, polydispersity, and detection of aggregates off your list in one shot. Without skipping a beat, you’ll know if your AAV, nanoparticle or protein is good to go.

Want more info?

Want to learn more about how Stunner makes it easy to get to know your protein sample?