Payload quantification

The problem

Nanoparticles are made for a lot of different payloads – RNA, DNA, protein or practically any other active pharmaceutical ingredient. When it comes to payload quantification most solutions need complex assays to get around lipid nanoparticle (LNP) and nanoparticle turbidity. Specifically for RNA-LNPs samples are so cloudy that fluorescent dyes are used for quantification, but tricky dye-based methods are nobody’s favorite for nanoparticle analysis.

UV/Vis absorbance has been around forever as a fast, easy and low-volume method for RNA quantification in purified samples, but the cloudiness of nanoparticle samples stops most UV/Vis spectrophotometers from getting accurate nanoparticle analysis readings.

The right tool for the job

Stunner pairs up intelligent, short pathlength UV/Vis with high-throughput RADLS to simultaneously quantify total RNA and size, even on cloudy nanoparticle samples. With our suite of nanoparticle analysis apps, Stunner quantifies total payload concentration for RNA, DNA, protein, or small molecules – without the need for any reagents, standards, or fluorescent dyes. For one-of-a-kind nanoparticles, Stunner can learn and remember everything about the UV/Vis absorbance of a particle – from carrier to payload. On the same 2 µL sample, Stunner delivers RADLS data to get the particle concentration, size and PDI data you need. All this is done in under 2 minutes per sample in our 96-well microfluidic plate.

The proof

Spotlight your payload

Turbidity is no longer a dealbreaker for UV/Vis spectrophotometry thanks to Stunner’s Unmix app algorithms. Cut through turbidity and check out just the absorbance signal from your payload.

Dye-free quant straight up

When you’ve got lots of buffers, formulations and RNA constructs to test, you don’t want to spend the next couple of days in the lab. Stunner gets you payload quantification on up to 96 LNP samples in around 2 hours so you’re never waiting around for those complicated assays to finish It’s the simplest possible assay for total RNA quant.

Stunner

Stunner is the only nanoparticle payload quantification system that pulls together UV/Vis concentration, rotating angle dynamic light scattering (RADLS) and multi-angle static light scattering (MALS) data from the same 2 μL sample. Nail down your lipid nanoparticle quality by knocking particle concentration, size, mass, detection of aggregates and total RNA off your list in one shot. Without skipping a beat, you’ll know if your nanoparticle is good to go.

Meet Stunner