Lipid nanoparticles (LNPs) need to be optimized for lipid chemistry, payload encapsulation, and size. Once they’re optimized, you need to make enough to characterize and test them. Making the right-sized LNPs using conventional techniques is tedious because you first have to run multiple experiments to find the instrument settings that make the correct LNP size. This usually takes loads of single-use consumables and tons of time. When you finally get to the right size, you have to switch to a different instrument to make enough of them for all your characterization and functional tests. This means re-optimizing the instrument settings all over again to get large enough volumes.
The right tool for the job
Nail your LNP size and get high encapsulation
No matter what total flow rate (TFR) you need and what chemistry is used, from lipids (cationic; ionizable), buffers, to the type of nucleic acid payload (PolyA, ssDNA, or mRNA), Nunchuck makes it fast and easy to formulate monodispersed, size-tunable LNPs with >85% encapsulation efficacy.
It’s critical to ensure batch-to-batch reproducibility and homogeneity throughout the formulation volume. At any selected total flow rate (TFR), Nunchuck can make from 0.5-200 mL for characterizations with high reproducibility and no user-to-user variability from batch to batch.
Want more info?
Want to learn more about how Nunchuck makes perfectly-sized LNPs?