Single Particle Interferometry
Score high resolution sizing of single lentivirus particles & EVs
See the small stuff
In the world of biologics and gene therapy, when individual particles get too small to be seen by normal optical techniques, single particle interferometry is the go-to technique for one-by-one detection. Interferometry – specifically, interferometric reflectance imaging - is a unique way to measure mass or size of particles attached to a surface. When a particle latches on to a surface there is a change in how the light scattered by the particle interacts with light reflected from the surface – making interference patterns that tell you a lot about the particle. If you use interferometry when looking at individual particles, it gives you resolution down to the nanometer and can tell you a lot about gene therapy vectors – like lentivirus or exosomes.
Capture your targets
To get proteins and viruses to stay still, antibodies are used to capture target molecules at the surface of a silicon dioxide layer on top of a silicon layer.
Light ‘em up
A light source shines down on everything – and then gets reflected back up. The whole key to interferometry is that light scattered by a bound particle will interact with the light reflected by the surface, creating interference patterns that are dependent on the size and composition of the particle. Pick that up by a camera, and you can image particles one-by-one.
Make it real
If you know enough about your sample, you can translate these interference patterns into high resolution, single-particle size data on particles so tiny that they normally can’t be seen.
Ready for more?
Scientists now have the perfect solution for sizing lentiviruses with an instrument that sports super high resolution single particle interferometry. Have a question or ready to find out more?