Stunner

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Stunner

The ultimate gene therapy tool

Combine and conquer

Stunner is the only system that pulls together UV/Vis concentration, Dynamic Light Scattering (DLS) and Static Light Scattering (SLS) data from the same 2 μL sample. Dig in to your AAV by bringing protein and ssDNA concentrations together with light scattering to get the total capsid titer and empty/full ratio. Nail down your protein quality by knocking concentration, hydrodynamic size, polydispersity, and detection of aggregates off your list in one shot. Without skipping a beat, you’ll know if your AAV or protein is good to go. Get brochure.

AAV Capsid Titer

AAV Empty/Full Ratio

LNP Total RNA Quant

Aggregation

Concentration

Sizing & Polydispersity

B22 & kD

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Teeny sample, tons of info

Just load 2 μL of sample in a Stunner plate — don’t bother with sample prep or dilution. Each microfluidic circuit has two fixed pathlengths built into it to cover a wide dynamic range of 0.03-275 OD. If you’re dealing with a full plate, get 96 concentration measurements in 10 minutes — add on DLS sizing and have both done in an hour. For even heavier workflows, hook it up to your favorite robot to add more oomph.

Look your AAV up and down

Drop in your AAV and before you can blink, DLS & SLS figure out how many intact capsids you have or if a bunch of aggregates are screwing things up. See empty/full ratio, total protein and total ssDNA in about a minute with UV/Vis. Don’t worry about extinction coefficients or overlapping spectra – Stunner does all the math for you.

Know your AAV inside out

Get to the numbers you actually want – titers. Stunner bridges DLS and UV/Vis data to tally up how many full and empty capsids are present, and how much extra protein and DNA is left over. Take your cleaned up AAV and sneak a peek down to 1012 vg/mL. In just one assay, Stunner’s dye-free, label-free, standard-free, hassle-free workflow tells the whole titer story.

Titer for any serotype

Every AAV is different, but Stunner figures them all out. Pick a preloaded serotype or feed in the specs for your virus and get answers in seconds. Now you can check capsid titers and empty/full ratio as often as you want.

Aggregates ruin everything

Globs of AAV can stand between you and high-quality data for pretty much every assay. DLS checks if your AAV is monodisperse so the rest of your process doesn’t get tripped up by aggregates.

See through the fog

Cloudy solutions of LNPs hang up other techniques but Stunner’s short pathlengths teamed with DLS and UV/Vis get you the answers you need. Cut through all that turbidity with Unmix and check out just the absorbance signal from RNA.

Get the skinny on proteins

Stunner pulls rank on other systems because it’s way, way more than just DLS. It lets you get a grip on what storage, agitation or a change to your process or formulation does to your protein. See if there’s any aggregation, measure the hydrodynamic size, grab polydispersity when you need to check uniformity, and get the exact concentration while you’re at it.

Max out your biologic

Stunner is the only system out there that can measure biologics at high-throughput and high concentration. It’s got dynamic range that covers from 0.02 mg/mL to 200 mg/mL (mAb), so run any protein without ever having to dilute again. Stop the madness of running just one protein at a time — and cleaning up afterwards.

Ridiculously good data

Get spot-on precision within 1% and accuracy within 2%. Using two fixed pathlengths, Stunner gets you jaw-dropping data at both low and high protein concentrations and nails the expected concentrations every time. With the smallest sample size, the highest throughput and crazy accurate results, Stunner is hands down the best tool out there for protein quantification.

Double down with B22 and kD

On the fly data tells you if your sample’s in good shape, or if aggregation is in the cards. Because the concentration of each sample in the series is measured in real time with DLS, you get the most accurate values without breaking a sweat.

Brew your own

Pin down what else is going on with your sample, or just check out its other characteristics. Pick a few wavelengths, a background subtraction method, and create your favorite equation. Stunner’s Homebrew tool is wide open, so you can create apps just the way you like them.

Accuracy you can show off

Stunner has shocking accuracy and wants to prove it. Using the Fundamentals tryptophan standards, the accuracy, precision and linearity of the instrument can be proven at any time – at the protein-relevant 280 nm wavelength and OD range of 20-225. Be confident about every sample with data that leaves no room for doubt.

Downstream ready

Smash through the USP & Ph. Eur. UV/Vis requirements for absorbance accuracy, precision, linearity, wavelength accuracy, stray light and resolution with performance verification measurements of independently certified NIST standards. Stunner is ready when you are to get validated and make the move into QC.

Put it on lockdown

GLP labs don’t sweat it. Stunner’s software hooks labs up with 21 CFR Part 11 compliant features. We’re talking password protection, electronic signatures, full audit trail — the whole package.

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