Buffer exchange & sample preparation
Buffer exchange and sample concentration is part of everyday life for scientists working with antibodies and recombinant proteins. Whether it’s for sample prep, preparing for labeling or characterization, or developing formulation screening and optimization strategies for a biologic, the process is monotonous, labor-intensive, time-consuming, and prone to error.
Conventional buffer exchange methods like dialysis and centrifugal filtration suffer from inconsistency in results and are often hard to manage in larger numbers. Sample concentration is usually an additional time-consuming step. Most methods require hands-on time, taking researchers away from other critical work.
The right tools for the job
Buffer exchange with Unagi
Whether you want to exchange 1 protein into 8 buffers, 8 different proteins into 1 buffer or anything in between, it only takes minutes to load up Unagi and it's off to the races.
Fast formulation screening and optimization with Big Tuna
Screening for the optimal pH for your antibody is easier than ever before. It only takes 30 minutes to set up and you’ll be running your stability measurements in no time.
Dialing in your formulation with Big Tuna
Buffer exchange and concentration are two of the most annoying things you have to do before you can work with your sample, even for just a handful. It takes forever, you physically have to be there and just when you think it’s done, you’re tweaking it again, even for a handful of samples. Unagi is the first completely hands-free benchtop buffer exchange solution that’s made to take this not-so-fun task off your plate — so you can be anywhere else instead of tied to your bench.
Buffer exchange and sample concentration are the time consuming, hands-on chores you must deal with before all the things you really want to do. Big Tuna is a fully automated, plate-based buffer exchange platform that uses a modified UF/DF method and gentle mixing to buffer exchange, concentrate, and clean up proteins, nucleic acids, or gene therapy vectors like AAVs and LNPs. Skip the slow and manual ways of prepping samples to free yourself up for other critical work.
Want more info?
Want to learn more about how Unagi and Big Tuna free you up from buffer exchange?