Adenovirus titer & empty/full ratio
Adenovirus (AdV) vectors are extremely versatile and at the heart of many gene therapies and vaccines in development. When making or working with adenovirus, knowing your capsid titer, empty/full ratio, and if things are aggregated is a big deal. After ultracentrifugation or chromatography steps the titer, empty/full ratio and purity of AdV samples or fractions are needed for making quick decisions on what to do next.
UV/Vis absorbance at 260 nm (A260) is often considered the fastest analytical method for a quick check of AdV titer – but all the work required to denature AdV easily makes it a 30 minute assay. On top of that, A260 results get thrown off by protein, DNA contamination, or just about anything else that absorbs. Other more technical methods exist, but are too slow, hands-on or sample-hungry, and none are ready for a quick, easy check of your adenovirus sample.
The right tool for the job
Combine UV/Vis with the DLS & SLS results and Stunner delivers quick, easy answers for total and full capsid titer that’s robust against contamination, all from just 2 µL sample.
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Want to learn more about how Stunner gives dye-free, standard-free, hassle-free AdV titers and empty/full ratios?