AAV reformulation & concentration

The problem

AAVs are often produced in dilute volumes that need to be cleaned up and concentrated. Tackling this process with centrifugal filtration devices or one at a time flow filtration instruments is labor intensive, especially at high sample numbers and volumes. Viral vectors like AAV need rapid clean up and buffer exchange workflows that don’t compromise the integrity of the sample and have high recovery.

The right tool for the job

Big Tuna is a fully automated buffer exchange system that provides a more uniform sample handling approach and degrees of process control that you just can’t get with manual methods. Big Tuna uses a modified ultrafiltration/diafiltration (UF/DF) method to exchange and concentrate AAVs and other viral vectors. Big Tuna monitors the sample volumes during the run, adds back the filtered volume and gets your samples to the right percent exchange and concentration every time.

automated_buffer_exchange_unfilter_only

Big Tuna uses a plate-based consumable called the Unfilter. All Unfilters use a regenerated cellulose membrane which is ideal for viral vectors because it minimizes non-specific binding. They come in 2 formats, 24-well (0.45 – 8 mL) or 96-well (100 – 450 µL). The Unfilter 96 is available with molecular weight cutoffs of 3, 10, 30, and 100 kDa. The Unfilter 24 has 10, 30, and 100 kDa MWCOs. The higher molecular weight cutoffs are perfect for large viral vectors and Unfilter 24 can concentrate up to 48 mL down to 8 mL.

2plates

The proof

Crank up the concentration

AAVs need to be concentrated up to do formulation work, for storage, or for characterization. Big Tuna can concentrate these up to 100x - from 48 mL to 450 µL. The 24-well, 30 kDa Unfilter keeps the AAV above the membrane while rapidly removing unwanted volume and handling the AAV with care.

large-get-concentrated-45
Get your AAV to its happy place

AAV stability can be impacted by changes to buffer pH, salts, and ionic strength. When Big Tuna partners up with Uncle, a wide range of these conditions can be studied without using tons of sample. Big Tuna can gently exchange your AAVs into various conditions without disrupting the capsids. Then Uncle can come in and assess the AAV capsid stability in those conditions based on the temperature that the genome begins to eject.

Get your AAV to its happy place_R2
Big Tuna front_crop

Big Tuna

Buffer exchange and sample concentration are the time consuming, hands-on chores you have to deal with before all the things you really want to do. Big Tuna automates exchange to formulate, concentrate and clean up proteins or gene therapy vectors like AAVs, LNPs, and VLPs — with just 30 mins of setup time. Skip the slow and manual ways of prepping samples to free yourself up for other critical work.

Want more info?

Want to learn more about how Big Tuna handles your AAV and gene therapy molecules with ease?