LNP quantification & sizing
Nanoparticles are made for a lot of different payloads – RNA, DNA, protein or practically any other active pharmaceutical ingredient. When it comes to quantifying payloads most solutions need complex assays to get around LNP and nanoparticle turbidity. Because samples are so cloudy, fluorescent dyes are used for quantification but tricky dye-based methods are nobody’s favorite.
UV/Vis absorbance has been around forever as a fast, easy and low-volume method for RNA quantification in purified samples, but the cloudiness of nanoparticle samples stops most UV/Vis spectrophotometers from getting accurate readings. Beyond quantification, keeping particles the right size is key to their success, so building up a stash of sizing data is everyone’s goal. But when you want size data on every sample, many dynamic light scattering (DLS) instruments are stuck measuring samples one at a time.
The right tool for the job
Spotlight your payload
Measure it all
When you’ve got lots of buffers, formulations, and RNA constructs to test you want size data on everything. Stunner reads up to 96 LNP samples in just 1 hour so you’re never waiting around for those old school, one-by-one instruments to finish. When even higher throughput is needed, Stunner is automation-friendly with SBS format plates.
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Want to learn more about how Stunner delivers quant and sizing for any nanoparticle?