LNP clean-up & concentration

The problem

After lipid nanoparticles (LNP) are formed, organic solvents like ethanol need to be removed quickly to maintain drug encapsulation and LNP integrity. Dialysis is often used but takes longer and requires a secondary concentration step to reduce the sample volume.

The right tool for the job

Big Tuna is a fully automated buffer exchange system that provides a more uniform sample handling approach and degrees of process control that you just can’t get with manual methods. Big Tuna uses a modified ultrafiltration/diafiltration (UF/DF) method to quickly clean up, concentrate and exchange LNPs. Big Tuna monitors the sample volumes during the run, adds back the filtered volume and gets your samples to the right percent exchange and concentration every time. The organic solvents that LNPs typically start in flow rapidly and Big Tuna has the ability to make the right adjustment, so wells won’t run dry.

automated_buffer_exchange_unfilter_only

Big Tuna uses a plate-based consumable called the Unfilter. All Unfilters use a regenerated cellulose membrane that reduces non-specific binding. They come in 2 formats, 24-well (0.45 – 8 mL) or 96-well (100 – 450 µL). The Unfilter 96 is available with molecular weight cutoffs of 3, 10, 30, and 100 kDa. The Unfilter 24 has 10, 30, and 100 kDa MWCOs. The 24- well, 100 kDa Unfilter is best for processing high volume LNP samples at fast rates.

2plates

The proof

Big Tuna is really good at nanoparticle concentration and clean-up while also maintaining payload encapsulation. A typical nanoparticle concentration and cleanup run can be set up in under 30 minutes and can be finished in as little as 90 minutes.

LNP clean up & concentrate table
Big Tuna front_crop

Big Tuna

Buffer exchange and nanoparticle concentration are the time consuming, hands-on chores you have to deal with before all the things you really want to do. Big Tuna automates exchange to formulate, concentrate and clean up proteins or gene therapy vectors like AAVs, LNPs, and VLPs — with just 30 mins of setup time. Skip the slow and manual ways of prepping samples to free yourself up for other critical work.

Want more info?

Want to learn more about how Big Tuna can help you prep your lipid nanoparticles?