AAV titer & empty/full ratio
Quick and efficient quantification of adeno-associated virus (AAV) titers and AAV empty/full ratios is a major challenge in development and manufacturing. Decisions often have to be made based on AAV titer or empty/full ratio and answers need to be fast, easy, and require minimal sample volumes.
AUC, TEM, HPLC, ELISA or PCR-based methods each give you some of the info on a sample, but none of them deliver results quickly, easily or from low sample volumes. AUC is often called the gold-standard measurement, but requires a ton of time, sample, and specialized equipment. ELISA and PCR are solid workhorse techniques but take hours and require development and optimization for each AAV vector.
The right tool for the job
UV/Vis spectroscopy sees the total amount of protein and DNA present in a sample, and the ratio of protein and DNA gives a read on the empty/full ratio for purified AAV. When Stunner combines UV/Vis with DLS & SLS results, there’s enough info to measure the scattered light from capsids, and to know how many of those capsids are likely full. That gets all the way to determining total AAV titer, full capsid titer, and the empty/full ratio of a sample. If any excess DNA or protein is seen by UV/Vis, that’s called out as free & aggregated DNA or protein in a sample.
Want more info?
Want to learn more about how Stunner gives dye-free, standard-free, hassle-free AAV titers and empty/full ratios?