Extraction & purification QC
Extracted DNA and RNA samples can be freaking messy, but downstream assays and applications need pure, high-quality nucleic acids. Analytical methods that only look at either quantification or quality don’t help you decide if a sample is good to go or if it needs more purification. Fluorescent dye-based methods are classic examples – they quantify DNA and RNA, but don’t give any information on purity.
UV/Vis absorbance is the classic way of quantifying nucleic acids and looking at RNA and DNA quality using the A260/A280 and A260/A230 ratios. These ratios provide limited insight, which makes it hard to make decisions about a given sample. Plus, cuvette-based spectrophotometers take a lot of volume or need sample dilution, while pedestal and fibrette-based systems typically measure samples one-by-one with extra steps between measurements – all just wasting a lot of time.
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