Extraction & purification QC
Extracted DNA and RNA samples can be freaking messy, but downstream assays and applications need pure, high-quality nucleic acids. Analytical methods that only look at either quantification or quality don’t help you decide if a sample is good to go or if it needs more purification. Fluorescent dye-based methods are classic examples – they quantify DNA and RNA, but don’t give any information on purity.
UV/Vis absorbance is the classic way of quantifying nucleic acids and looking at RNA and DNA quality using the A260/A280 and A260/A230 ratios. These ratios provide limited insight, which makes it hard to make decisions about a given sample. Plus, cuvette-based spectrophotometers take a lot of volume or need sample dilution, while pedestal and fibrette-based systems typically measure samples one-by-one with extra steps between measurements – all just wasting a lot of time.
The right tool for the job
Lunatic makes nucleic acid quantification and QC by UV absorbance a no–brainer. All you need is 2 μL and 5 -10 minutes to measure up to 96 samples. The fixed pathlength microcuvettes in each of the 96 microfluidic circuits per plate means you can skip the sample prep and cleaning that bogs down other methods. All you do is load up your samples and get kick-ass results.
Get all the ratios
UV/Vis spectroscopy is one of the fastest and easiest techniques to quantify DNA or RNA. While doing crazy accurate quantification, Lunatic has all the tools to check on RNA and DNA quality including the presence of co-absorbing impurities, and the up-front reporting for the A260/A280 and A260/A230 ratios that you’re used to seeing.
Lunatic puts the full potential of UV/Vis spectroscopy to work with Unmix applications that analyze complex UV/Vis spectra to call out the signal from nucleic acids and common impurities. This allows for quantification of DNA and RNA even in samples that have some problems, and makes it much easier to troubleshoot RNA and DNA quality issues versus looking at just absorbance ratios.
Lunatic makes batch quantification of protein, DNA and RNA a no-brainer. All you need is 2 μL and 10 minutes to measure up to 96 samples. Run them straight-up, even at high concentrations, without ever diluting. Lunatic gets biologics and genomics UV/Vis quantification on the money every time. Just drop, load and read.
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