Leprechaun takes the guesswork out of your lentivirus production
Blog
Lentivirus production and purification can feel like navigating a maze. It is a complex, multi-step process following a standardized framework of harvest, clarification, filtration, chromatographic separation, and final polishing. Each step is essential for ensuring a high yield and purity of intact lentivirus, but lentivirus production can be both time-consuming and costly. That’s why optimizing each step is crucial.
Struggling to see the whole lenti picture?
There are several established methods for performing lentivirus QC: ELISA ensures the presence of the viral capsid, qRT-PCR confirms the presence of RNA, and cell-based transduction assays confirm the functionality of the final product. However, each method has its limitations. They often lack information on virus structural information (all), fail to differentiate capsid and soluble p24 (ELISA), or take too long to deliver results (transduction assay). Effective process optimization hinges on the ability to accurately quantify the content of lentivirus preps before and after each process step. The inabililty of established analytical methods to provide information on crude samples limits their suitability for process development optimization. Moreover, these analytical methods focus exclusively on lentiviral components within a sample. As the cell therapy market evolves, there is a growing need to monitor the titer and functional impact of non-viral contaminants within the lentivirus prep, which may interfere with viral function.
Streamline lentivirus production and purification with Leprechaun
With Leprechaun, you no longer need to worry about sample purity. Developed for total lentivirus analysis, Leprechaun helps streamline QC and the development of the lentivirus production process. The platform provides complete biophysical characterization of any lentivirus product by measuring intact lentivirus titer through detection of viral capsid and RNA, while also quantifying the concentration of contaminating particles, such as extracellular vesicles (EVs).
Track down the good stuff at every stage
By monitoring intact lentivirus titer and contaminant concentration in parallel, before and after each process step, you can optimize every step in the purification process to ensure the most structurally intact lentivirus particles. Plus, with our Leprechaun kits, you can identify which batches have the highest quality material at harvest with the RNA kit, delve into purity analysis with our contaminant kit, or customize the assay or capture marker using our Flex Kit. Leprechaun is compatible with both VSV-G and non-VSVG pseudotyped lentivirus.
Conclusion
With Leprechaun, we take the guesswork out of your lentivirus production, giving you clear, comprehensive insights every step of the way. So, why not streamline your lentivirus production process and ensure the highest quality lentivirus particles with Leprechaun? Your research deserves nothing less.
Leprechaun
Leprechaun is the only system that hunts down titer and structure for viruses and exosomes without worrying about sample purity. Triple-check lentivirus particles to be sure they are the right size, have the right structure and contain RNA – in both crude and pure samples. Uncover exosome concentration and phenotype in everything from cell culture to biofluids. Make your own luck and follow Leprechaun straight to the viral titer or exosome concentration you’ve been looking for, without the noise from trickster particles throwing you off the trail.
