High-throughput, low-volume adeno-associated virus titer quantification and empty/full ratio
Adeno-associated virus (AAV) quantification and empty/full ratios are a major challenge for developing and manufacturing gene therapy vectors. AUC, TEM, ELISA or PCR-based methods each give you some of the info on a sample, but none of them deliver results quickly, easily or from low sample volumes. TEM and AUC are gold-standard measurements, but require a lot of time, sample, and specialized equipment. ELISA and qPCR are solid workhorse techniques but take hours and require development and optimization for each AAV vector.
Stunner determines sample titers quickly and easily so it’s possible to check capsid titer and empty/full ratios as often as you want. A read on Stunner before a PCR method takes the guesswork out of choosing a dilution factor or can be a quick check for when the slower PCR run is overkill. For those experiments, a quick check of sample titer on Stunner spots aggregates that will mess up your results.
Using Stunner to judge capsid titers gives a faster and fuller picture of your sample that avoids slow and expensive assays like ELISA.