Leprechaun
The ultimate tool for single-particle biophysical characterization of lentivirus & exosomes from crude or purified samples
Find your pot of gold with interferometry and fluorescence
Leprechaun uses antibody capture, interferometric sizing, and 3-channel fluorescence imaging to rapidly capture, count, size and characterize lentiviral and extracellular vesicle particles from crude or purified samples. It delivers single-particle sizing plus surface protein or cargo detection for complete particle analysis on up to 16 samples per run. Get brochure.
- Lenti quality from crude samples: Measure the size and purity of lentivirus directly from in-process samples to catch poor-quality batches early in development.
- Exosome results from biofluids: Detect and characterize exosomes straight from serum, CSF or other biofluid — no isolation step needed — so rare populations aren’t lost.
- Custom capture: Use your own antibodies to capture specific viral vectors or EV subtypes for tailored biomarker or cargo analysis.
- Sub-population analysis: Co-localize fluorescence markers to see which particles carry payload, have intact capsid, or express specific surface proteins.
- Particle sizing: Determine particle size and size distribution to verify batch consistency and cargo integrity.
- Contaminant detection: Quantify free pP24 capsid or co-purifying EVs to fine-tune purification and improve QC.
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Video
Check out EV characterization with Leprechaun
Watch lentivirus titer, structure, and RNA content with Leprechaun
Follow the rainbow
Leprechaun characterizes vectors like lentivirus and exosomes — on up to 16 samples at a time. The Luni consumable is where 1-25 μL of your vector is captured, sized, and then the structure of every particle is identified using immunofluorescence. Out-of- the-box kits with step-by-step protocols deliver answers in just a few hours.
Any lenti, anytime, anywhere
Rack up complete biophysical characterization of each lentivirus particle at every stage of the production process. Capture lenti down to 1x107 particles/mL using our antibodies or yours, then size them up to sort out individual viruses from aggregates. Confirm how many of your viruses have a capsid with fluorescent antibodies while RNA dye lights up the ones that have that critical nucleic acid on board. Leprechaun can tackle any lentivirus, whatever the flavor.
Track down the good stuff
Counting up how many of your lentivirus contain RNA at the first step of your process is tricky with all the cellular junk in upstream samples. Leprechaun’s RNA Kit pulls back the curtain at harvest to reveal which batches have the highest quality starting material. Move ahead with only the best preps and leave the trash behind.
Sniff out contaminants
Dig into the details to see if your clean-up steps are doing their job with the Lentivirus EV Contaminant Kit. Ensure your batch isn’t full of undercover EVs pretending to be virus. Figure out how much p24 is inside viral particles and how much is roaming free as a soluble contaminant. For the first time you can monitor titer, structure and purity throughout your whole process.
Explore your exosome
Getting a total concentration for your exosomes is tricky and confirming that your cell line made what you want is crazy hard. Leprechaun gets exosome concentration in a jiff by grabbing them by a slew of surface proteins, sizing them up and doubling-down with fluorescence to confirm all the right proteins are there. Whether your markers are internal or external Leprechaun gives you colocalization info on up to four proteins, all from a few μl of sample without the need for purification.
Size up your EVs
Get the measure of even the smallest vesicles with Leprechaun. Single particle sizing down to 35 nm means no EV is off limits. From exosome to exomere Leprechaun lets you size, count and phenotype the small stuff so you can get the full picture of what’s in your sample.
What a flex
For unique situations you can customize the assay using your own detection antibodies or change up the capture marker using a Flex Kit. Look at any marker, in any sample, without the need to purify.
Cut to the chase
Zero in on particle concentration, size and structure with easy to use, automated analysis software. Have faith in your results with auto disabling of outliers and background removal. Leprechaun Analysis gives instant results for your lentivirus or EVs, whatever the structure or subpopulation. If you need to tweak things, customizable size ranges and fluorescent cut-offs let you tune to your taste.
Tech
Specifications
| Instrument | Specification |
|---|---|
| Dimensions | 23.4 cm W x 47.8 cm D x 35.2 cm H; 20.5 kg |
| Computer | Separate computer with Windows 11 included |
| Electrical | Input voltage: 110-220 V AC, 50-60 Hz, Max power: 72 Watt |
| Detection method | Cooled Scientific CMOS image sensor |
| Approval | CE, TUV |
| Interference Reflectance Microscopy | |
|---|---|
| Light source | 415 nm LED |
| Size range | 35-200 nm |
| Sizing accuracy (% error) | ≤5% |
| Fluorescence Microscopy | |
|---|---|
| Light sources | LED: 470 nm (Blue), 567 nm (Green), 623 nm (Red) |
| Excitation and emission filters | Blue: 465-495 nm (ex); 505-530 nm (em) Green: 543-568 nm (ex); 580-608 nm (em) Red: 625-655 nm (ex); 665-725 nm (em) |
| Fluorescence intensity precision | ≤5% |
| Consumable | |
|---|---|
| Samples per Luni | 1 |
| Technical replicates per Luni | 3 or 6, application dependent |
| Lunis per run | 1-16 |
| Assay Specifications | |
|---|---|
| Sample compatibility | Lentivirus: From cell harvest to purified sample Exosomes: Cell culture media, plasma, serum, CSF, urine |
| Sample volume range | 1-25 µL diluted in supplied buffer |
| Titer dynamic range | Lentivirus: 1x107–5x108 vp/mL Exosomes:
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