Stunner

The only plate-based dynamic light scattering & UV/Vis tool built for biologics

Brochures

Conquer protein characterization with DLS & UV/Vis

Stunner delivers plate-based, high-throughput dynamic light scattering (DLS) and UV/Vis absorbance data from 2 µL of sample for up to 96 samples. Read UV/Vis during the same runs as DLS to pair up quantification and sizing of proteins, ADCs, peptides – you name it.

• Size & polydispersity: See whether your protein’s diameter is what you expect and how uniform it is — critical for screening, formulation, and QC.

• Aggregate detection: Flag unwanted particles and samples that have gone south, so you can fix issues fast.

• Protein concentration: Get extremely accurate quantification based on sample absorbance across a huge range from 0.03-275 mg/mL – while also seeing when impurities are lurking.

• Conjugate characterization: Gain clarity on ADC samples with DAR characterization that is ideal for high-throughput optimization.

• B₂₂ & k_D: Get early insight into colloidal stability based on protein self-interactions, helping predict protein stability and the impact of different formulations.

Dynamic light scattering size distribution and UV/Vis absorbance graphs generated by Stunner protein analysis equipment.

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Videos

Scope protein quant and quality with Stunner

Hi. I'm Ross and I'm here to show you a stunner, the best way to blast through quant and aggregation checks of your protein samples in no time. With Stunner, all you need is two microliters of sample, and in less than a minute, you've got dye free, label free, hassle free quant, and quality results. Andre to Rob. I need to measure the concentration of a bunch of protein samples I found in this strange alien world. Do you have a high throughput, low volume way to measure protein quantity and quality at the same time? Of course. Computer beam up those protein cells. Now that we have some samples, let me show you how to load them into Stoner's one of a kind ninety six well plate. When we load the sample into the input well, capillary action pulls it into the microfluidic circuit. First step, we click on the protein menu and select protein plus sizing. Then, we fill in an experiment name and hit next. Next, we can choose to populate each well manually, for example, to tell it that we have a buffer blank and a bunch of samples, or we can simply import and pull in all of the information from an Excel file. Once the plate is inside Stoner, it uses dynamic light scattering to look for aggregates and UV Vis spectrophotometry to measure concentration in the same experiment. But how does that work? Happy proteins tend to have a well defined size, but sometimes under certain conditions, they'll aggregate. These aggregated proteins don't function as well and can even be dangerous, especially for those proteins that are destined to be therapeutics. Dynamic light scattering, or DLS, identifies protein aggregates in solution by measuring how fast the light it scatters fluctuates. Small particles zip around quickly in solution and make the intensity of light change super fast. Larger ones move slower, and the light intensity changes slower too. Analyzing whether light intensity fluctuates quickly or slowly, that's the secret to DLS. Happy, intact, and homogeneous particles show up as a single narrow peak in the DLS results. Aggregated proteins, especially large aggregates, show up as multiple peaks, and on average have a larger diameter than the intact protein. That covers the quality question, but what about protein quant? Proteins have an absorbance peak in the UE VIS spectrum at two hundred eighty nanometers. Based on the a two eighty value, Stunner can determine the concentration of proteins from about zero point zero two up to two hundred milligrams per milliliter for a typical monoclonal antibody. But that's not all. While you're checking for aggregates, why not check for impurities too? Stundner's UV Vis is equipped with unmixed algorithms that can dissect an absorbent spectrum to look for what else might be lurking in there. You'll see if your a two eighty is impacted by turbidity, nucleic acids, EDTA, or other impurities, and have the power to quantify your protein despite those problems. That's how Stoner can make your protein quant and quality measurements hit warp speed. Computer, set a course for the next experiment. Right away, sir. Hey, aren't you gonna get me off this planet? Hello? Anybody? I can't believe they left me here.

Scope protein quant and quality with Stunner

Tap into colloidal stability with Stunner

Hi. Hey. Howdy. Oh, are we rolling? Hi. I'm Brian, and I'm here to introduce you to measuring collateral stability with Stunner, the ultimate quantification and sizing tool. Oh, yeah. Stunner is the only plate based platform that bundles UV Vis and light scattering to get you k d, b two two, concentration, and tons more deeds on your proteins from just two microliters of sample. When screening your formulations, you wanna make sure your proteins are in the right environment and check if they're at risk for aggregation by seeing how they interact with each other. Measuring k d b two two for a protein in different formulations lets you know if your proteins will continue to be happy hanging out side by side even when concentrations go up and things get crowded. The current methods use too much volume, can't keep up with high throughput needs, or don't measure concentration at the same time. Let's see how Stunner here gets it done. We'll start off by loading our Stunner plate. Just pipette in two microliters for each sample in a dilution series and the microfluidic channels pull your samples in. Once your sample is ready to be read, it gets pulled through two microcubets with fixed path lengths to ensure the most accurate and precise UV Vis concentration data and light scattering data from the smallest possible volume. Now let's jump into the Stoner software. We'll select the protein button and select the application for KD and B22. Give your experiment a name and we're set. Here we can create our plate layout either manually by adding in our blanks and samples or we can import from an excel file. Select whatever type of protein you have, the buffer you're working in, and stunner is ready to go. And now we just drop in our plate and stunner is gonna turn up KD and B22 in no time. In stunner's analysis software, KD and B22 are served up for you right from the start. Positive slopes in the KD and b two two data are what you wanna see and mean that your protein is in the right formulation. If slopes are negative, watch out since that means attractive self interactions are putting your protein at risk for aggregation. If you want, it's easy to drill down into the data and also grab underlying concentration and size info. Now that you've seen how you can easily measure k d b two two and look good while doing it, you're set for hassle free colloidal stability of your proteins. Guys, is anyone still here?

Tap into colloidal stability with Stunner

Catch ADC characterization with Stunner

Check out Unmix for proteins on Stunner

Steady. Steady. Oh. Hi. I'm Brian, and I'm here to introduce you to protein quant, contaminant, and aggregation detection on Stunner, the protein quantification, sizing, and aggregate detection workhorse. Stunner is the only plate based platform that bundles UV/Vis and light scattering to give you accurate protein quant, aggregation detection, and tons more deets on your proteins from just two microliters of sample, and flexes Unmix algorithms that read out contaminants from your results to give you insights as to what's hiding in the mix and to dish up next level accuracy. When working with proteins, aggregates and contaminants can be lurking in your samples, completely throwing off accuracy of your measurements and risking you sending bad candidates to the next step. So getting accurate measurements is crucial to avoid getting burnt down the line. But traditional methods don't account for other components in your sample that could be contributing to this absorbance, such as turbidity or unwanted impurities. Stunner takes that UV/Vis absorbance spectrum and uses intelligent unmixed algorithms to separate out different components contributing to that total spectrum. Unmixed not only tells us if we have impurities in our samples, but also gives us an accurate protein concentration by only measuring the absorbance at two hundred eighty nanometers that's coming from our protein. Let's see how Stunner rolls it all out. Oh, so that's what a cupcake is supposed to taste like. Good job, buddy. We'll start off by loading our Stunner plate. Just pipette in two microliters of each sample and the microfluidic channels pull your samples in. Once your sample is ready to be read, it gets pulled through two microcuvettes with fixed path lengths to ensure the most accurate and precise UV/Vis concentration data and light scattering data from the smallest possible volume. Jumping into Stunner software, we'll select the protein button and select the application for proteins, lysates plus sizing to unleash all of Stunner's skills for measuring concentration, impurities, sizing, and aggregation on each sample. Impurities that are identified in these samples are listed in the app description. For protein samples, we've baked in some of the most common impurities for Stunner's Unmix to separate out. If you're working with samples from a different source, no worries. Stunner has apps designed for different sample types and their most common contaminants. Now we give our experiment a name, and we're set. Here, we can create our plate layout either manually by adding in our blanks and samples, or we can import from an Excel file. Select your protein type or added protein from the Analyte pull down list and the buffer you're working in, and Stunner is ready to go. And now we're just gonna drop in our plate, and Stunner's gonna cook up quant and aggregate detection in no time. Here we go. Looks like the results are ready. Now that we've whipped up some delicious results, we can review them starting here at the overview page. Here, we can see the summary of each sample with the absorbent spectrum displayed for each well. Moving over to well details, we can chew on the details from each well individually. All replicates are grouped here, so we can do a quick reproducibility check on our samples by selecting the sample instead of an individual replicate. When individual wells are selected, we can keep an eye on contaminants that are present in that sample and how much they are influencing the absorbance at two hundred eighty nanometers. To unwrap the unmixed spectrum, let's start by looking at the black line, which is the total UV/Vis spectrum. The green line is the portion of the spectrum from protein in our sample, and the blue line is the contribution from our impurities. To assess the fit, the yellow line represents the deviation from an exact fit. Here we can see the concentration from traditional UV/Vis and Unmix are very similar, which tells us that the samples are pure with minimal contaminants contributing to the absorbance at two hundred eighty nanometers. Whereas for this sample, Unmix flags significant contamination and gives us a breakdown of each of the components to give us our true concentration value. Finally, along with the unmatched results, sizing results from DLS are also collected on the same sample. We can see the average size of each sample and also whether aggregates are hiding under the main course. Now that you've seen how you can easily measure quant and aggregate detection on Stunner, you're ready to pick your best of the batch in no time. That is just delicious. Okay. How does this one taste? Maybe you should just stick to science. No.

Check out Unmix for proteins on Stunner

UV/Vis IgG absorbance curves showing protein concentration measurement with DLS-enabled analysis.

Ridiculously good data

Get spot-on precision within 1% and accuracy within 2%. Using two fixed pathlengths, Stunner gets you jaw-dropping data at both low and high protein concentrations and nails the expected concentrations every time. With the smallest sample size, the highest throughput and crazy accurate results, Stunner is hands down the best tool out there for protein quantification.

Get the skinny on proteins

Stunner serves up spot-on light scattering data for proteins, so you can compare samples head-to-head based on size, intensity, mass and number. Measure the hydrodynamic size, grab polydispersity when you need to check uniformity, and watch your replicates stack up right on top of each other.

DLS intensity and mass distribution plots illustrating nanoparticle sizing and protein aggregation profiles.

Dynamic light scattering graphs identifying large protein aggregates using high-sensitivity DLS instrumentation.

Track down the big boys

When it comes to the quick and easy detection of aggregates, Stunner pulls rank. See your monomer based on mass, then switch over to intensity to spot even the tiniest bit of aggregation. QC your protein at every step of your process and catch aggregation before it becomes a bigger problem.

Decode any ADC spectra

Measuring ADCs by UV/Vis gets tricky when drug spectra interfere in the UV range. Stunner’s Unmix separates ADC absorbance into the spectra from your antibody and from your drug or linker – even for overlapping spectra. Decode your ADC spectra automatically for a simple, high-throughput method to quantify antibody concentration, conjugate concentration and DAR.

Colloidal stability screening graphs measuring protein interactions and repulsive forces using DLS.

Feel the attraction

Stopping aggregation in its tracks is critical. Stunner deals out B₂₂ and k_D, indicators of a potentially looming problem, in one simple experiment. All you do is create a quick dilutions series, load it on a plate and you’re good to go. Stunner checks the exact concentrations while light scattering figures out if your protein is attractive (negative slope) or repulsive (positive slope) to itself.

Know your AAV inside out

Get to the numbers you actually want — titers. Stunner bridges DLS and UV/Vis data to tally up how many full and empty capsids are present, and how much extra protein and DNA is left over. Take your cleaned up AAV and sneak a peek down to 10¹² vg/mL. In just one assay, Stunner’s dye-free, label-free, standard-free, hassle-free workflow tells the whole titer story.

Bar charts showing AAV particle concentration and packaging efficiency measured by DLS and UV/Vis protein analysis equipment.

Tech

Dynamic Light Scattering (DLS)

UV/Vis

What it does:

UV/Vis measures the absorbance of light in the UV and visible (UV/Vis) range of the spectrum which correlates directly with the concentration of proteins, peptides, nucleic acids and other small molecules.

Why it matters:

UV/Vis can quickly measure protein or nucleic acid concentrations. Plus, Stunner’s powerful Unmix deconvolution algorithms can separate the absorbance from different molecules to quantify components from a mixture or conjugate, e.g., protein and DNA or an antibody and its conjugated drug.

Static Light Scattering (SLS)

Learn more

Webinars

View all resources

Sound the alarm on aggregation for proteins, peptides, and ADCs on Stunner

Kevin Lance, Director, Product Management, Unchained Labs

Decoding ADC spectra for DAR measurements at warp speed on Stunner

Kevin Lance, Director, Product Management, Unchained Labs

Rock mAb and ADC characterization with Stunner

Brian Haldeman, Product Manager - Stunner, Unchained Labs

Bust out quick, low-volume protein characterization on Stunner

Brian Haldeman, Product Manager - Stunner, Unchained Labs

Hit go for fast, low-volume protein characterization on Stunner

Brian Haldeman, Product Manager - Stunner, Unchained Labs

Spot-on, standard-free AAV titer and characterization with Stunner

Kevin Lance, Director, Product Management, Unchained Labs

Lose the lag with high-throughput and flexible ADC analysis on Stunner

Kevin Lance, Market Director, Unchained Labs

View all resources

App Notes

Decoding ADC spectra for DAR measurements at warp speed with Stunner

Conjugate, clean-up, & characterize ADCs on Unagi & Stunner

Have it all with protein quant & sizing on Stunner

Dive deep into Stunner’s light scattering

Take a peek inside your AAV capsid with Stunner

Spot-on UV/Vis accuracy with Lunatic & Stunner

Technology

Rotating Angle Dynamic Light Scattering (RADLS)

UV/Vis

Software

Download Stunner Software

Specifications

Biologics Viral Vectors

Biologics

Stunner instrument specifications (Biologics)
Dimensions	37 cm W x 54 cm D x 33 cm H; 30.4 kg
Electrical	Universal input voltage 100-240 V AC, 50-60 Hz
Computer	Separate computer with Windows 11 included
Connection	USB, TCP/IP (Service)
Approval	CE, FCC, CSA
Regulatory compliance	Optional 21CFRp11 software package USP and Ph. Eur. Performance verification standards

UV/Vis
Light source	Xenon flash lamp
Detectors	UV/Vis polychromatic spectrophotometer
Wavelength range	230-750 nm
Wavelength accuracy	≤400 nm: ±1 nm; ≥400 nm: ±2 nm
Spectral resolution	Better than 2 nm (toluene in hexane)
Absorbance precision (1 cm quartz cuvette)	<1 OD: ±0.005 OD st dev	1-2 OD: ±0.5% CV
Absorbance accuracy (1 cm quartz cuvette)	<1 OD: ±0.01 OD	1-2 OD: ±1%

DLS and rotating angle DLS
Light source	2 x 660 nm laser diodes
Detection	Avalanche photodiode module
Number of angles	1 (DLS), 5 – 30 (RADLS)
Angular range	30-42° 110–162°
Size accuracy	±2%
Minimum sample concentration	0.1 mg/mL lysozyme
Hydrodynamic diameter range	0.3–1000 nm
Molecular weight range	1 kDa – 10 GDa
Particle concentration range	10⁹ – 5x10¹³ particles/mL (dependent on particle size, determined on 80 nm beads)

Stunner plate specifications
Samples per plate	96 (12 x 8 microplate format)
Sample retention time	Up to 2 hours
Recommended sample volume	2 µL
Pathlength(s)	0.1 mm & 0.7 mm path
Measurement time for full plate	~10 minutes for UV/Vis only ~1 hour for UV/Vis and DLS (5 x 4s x 1 angle) ~2 h 15 min for UV/Vis and RADLS (5 x 1s x 7 angles)
Measurement range: OD 10 mm ng/µL dsDNA mg/mL ave protein mix	0.03–275 OD 10 mm 1.5–13750 ng/µL 0.03–275 mg/mL
Absorbance precision (10 mm pathlength)	<1 OD: ±0.01 OD st dev 1-200 OD: ±1% CV
Absorbance accuracy (10 mm pathlength)	<1 OD: ±0.02 OD 1-200 OD: ±2%

Viral Vectors

Stunner instrument specifications (Viral Vectors)
Dimensions	37 cm W x 54 cm D x 33 cm H; 30.4 kg
Electrical	Universal input voltage 100-240 V AC, 50-60 Hz
Computer	Separate computer with Windows 11 included
Connection	USB, TCP/IP (Service)
Approval	CE, FCC, CSA
Regulatory compliance	Optional 21CFRp11 software package USP and Ph. Eur. Performance verification standards

UV/Vis
Light source	Xenon flash lamp
Detectors	UV/Vis polychromatic spectrophotometer
Wavelength range	230-750 nm
Wavelength accuracy	≤400 nm: ±1nm; ≥400 nm: ±2 nm
Spectral resolution	Better than 2 nm (toluene in hexane)
Absorbance precision (1 cm quartz cuvette)	<1 OD: ±0.005 OD st dev	1-2 OD: ±0.5% CV
Absorbance accuracy (1 cm quartz cuvette)	<1 OD: ±0.01 OD	1-2 OD: ±1%

DLS and rotating angle DLS
Light sources	2 x 660 nm laser diodes
Detection	Avalanche photodiode module
Number of angles	1 (DLS), 5 – 30 (RADLS)
Angular range	30-42° 110–162°
Size accuracy	±2%
Minimum sample concentration	0.1 mg/mL lysozyme
Hydrodynamic diameter range	0.3–1000 nm
Molecular weight range	1 kDa – 10 GDa

Stunner plate specifications
Samples per plate	96 (12 x 8 microplate format)
Sample retention time	Up to 2 hours
Recommended sample volume	2 µL
Pathlength(s)	0.1 mm & 0.7 mm path
Measurement time for full plate	~1 h for AAV Quant (UV/Vis + DLS) ~2 h 15 min for Adeno Quant (UV/Vis + RADLS)
Measurement range: OD 10 mm AAV Quant Adeno Quant	0.03–275 OD 10 mm 10¹² – 5x10¹⁵ cp/mL 10⁹ – 5x10¹⁴ cp/mL
Absorbance precision (10 mm pathlength)	<1 OD: ±0.01 OD st dev 1-200 OD: ±1% CV
Absorbance accuracy (10 mm pathlength)	<1 OD: ±0.02 OD 1-200 OD: ±2%

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Biologics

Aunty

Stunner

Honeybun

Lunatic

Unagi

Stuntman

Big Tuna

Gene Therapy

Stunner

Aunty

Leprechaun

Lunatic

Unagi

Stuntman

Big Tuna

Cell Therapy

Leprechaun

LNPs

Sunscreen

Stunner AF

Sunshine

Unagi

Big Tuna

Sunbather

Exosomes

Leprechaun

Genomics

Lunatic

Automation

Stuntman

Big Tuna

Junior

Big Kahuna

Stunner

Brochures

Conquer protein characterization with DLS & UV/Vis

Videos

Scope protein quant and quality with Stunner

Scope protein quant and quality with Stunner

Tap into colloidal stability with Stunner

Tap into colloidal stability with Stunner

Catch ADC characterization with Stunner

Check out Unmix for proteins on Stunner

Check out Unmix for proteins on Stunner

Ridiculously good data

Get the skinny on proteins

Track down the big boys

Decode any ADC spectra

Feel the attraction

Know your AAV inside out

Tech

Dynamic Light Scattering (DLS)

UV/Vis

Static Light Scattering (SLS)

Learn more

Webinars

App Notes

Technology

Software

Specifications

Want a quote or a demo?

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Lunatic

Aunty

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