Nanoparticle Exploration & Optimization
The problem
Finding a promising therapeutic nanoparticle formulation during high throughput screening or when exploring novel nanoparticles is rarely an instant bullseye – there are a ton of particle performance characteristics to optimize. Homing in on the right size, PDI, encapsulation efficiency (EE%), and transfection efficacy can be a painstaking process, especially when you have to explore each set of prep conditions one-by-one. On top of all that, if you're using single-use consumables, the costs just keep piling up. Add in the need to make larger volumes, and you could end up on a different instrument entirely.
The right tool for the job
After finding promising formulations on Sunscreen, walk right over to Sunshine for protocol development, process optimization, and rapid scale-up. With walk-away automation, Sunshine can run up to 10 experiments in just 15 minutes and make tens of liters in a day. Sunshine uses the exact same methods and reusable Sunnies as Sunscreen and Sunbather so handover between platforms is totally seamless. Modify total flow rates and flow rate ratios and diluents with ease, producing particles from 40 nm to 200 nm.
If you don’t need in-line dilution, Sunshine Lite keeps all the other great Sunshine features in a slimmer footprint. For a quick one-and-done nanoparticle synthesis, Sunshine Single is on it. Once your ideal criteria for production, size, and encapsulation efficiency (EE%) are identified, seamlessly switch to Sunshine’s Continuous Mode and maintain that unwavering consistency through scale-up — with no limits on sample volume. When you’re ready for GMP production of your nanoparticles, Sunbather picks up right where Sunshine left off.
The proof
Flexibility and consistency to boot
Sunshine offers the ultimate flexibility for optimizing nanoparticle production and scale-up. Check out diverse flow rates, ratios, collection conditions, and mixing geometries with ease – with up to 10 automated experiments in a typical run. And when it comes to consistency, PDIs are typically lower than 0.2 and encapsulation efficiency >90%.
Hand it off
Forget pain and inconsistency as you move between stages in the nanoparticle development pipeline. Sunscreen, Sunshine and Sunbather use the same process technology and Sunnies (reusable glass microfluidic devices), at every stage. You can rely on complete transferability, continuity and scalability from screening and scale-up to GMP production of your LNPs, liposomes, polymer nanoparticles, or other novel therapeutic particle. This is a total game-changer for scientists in this space.
Sunshine
Sunshine is all about helping researchers create the perfect therapeutic nanoparticle. Automate your exploration of ideal flow rates, different mixing options, and the right dilution factors to keep them stable. When you’ve got everything just right, Sunshine can shift into continuous mode, making tens of liters in a day. Get process development and scale-up on one platform — who would have figured?
Want more info?
Want to learn more about how Sunshine helps you with LNP optimization?