Unagi
The fully automated benchtop UF/DF system for buffer exchange & concentration
Bite-sized buffer exchange & concentration with UF/DF
Unagi automates ultrafiltration and diafiltration (UF/DF) to exchange buffers, remove salts, and concentrate proteins, nucleic acids, viral vectors, or LNPs with high recovery. Process up to 8 samples in parallel with minimal hands-on time to get clean, consistent samples ready for any downstream assay. Get brochure.
• Parallel processing with flexible volumes: Run up to 8 samples in parallel, each from 0.5 to 8 mL, giving you the throughput and volume range needed for everything from early discovery to larger prep workflows.
• High recovery: Achieve ≥96% recovery during buffer exchange or desalting, preserving precious proteins, nucleic acids, viral vectors, and LNPs.
• Defined-volume concentration: Concentrate samples from 48 mL down to 8 mL with precise, automated final-volume control.
• Durable, low-binding consumables: Choose from regenerated cellulose (10, 30, 100 kDa MWCO) or polyethersulfone (30, 100 kDa MWCO) membranes to prevent sample loss, avoid absorption, and ensure clean separation.
• Hands-free operation: Load your samples and let Unagi run the entire UF/DF cycle unattended - freeing you from monitoring spin columns, filtration steps, or centrifuge cycles.
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Served up a’ la carte
Drop 1 to 8 of your samples into some Unas, twist them into the holder and boom, they’re ready to go. Each Una can handle from 0.5 to 8 mL of sample. They have regenerated cellulose (10, 30, and 100 kDa MWCOs) and polyethersulfone (30 and 100 kDa MWCOs) to make sure your samples stay put, don’t get absorbed, and the liquid passes right through — with ≥96% recovery.
Put in your order
Unagi is super easy to use — so everyone can get a taste. First choose the type of experiment you want to run. Then tell it what kind of molecule you’re exchanging, how much you have, and what buffer to exchange into. Last step is to input percent exchange and final volume if you want to concentrate your sample.
Get your fill
Now you just follow Unagi’s step-by-step instructions. Load up the tray of Unas filled with your samples in the front. Fill Falcon tubes with your buffers and plop them in on the left. Throw some tip racks in on the right and Unagi is ready to go. No joke, it’s that easy.
Let it roll
Hit start and let Unagi do its thing. Now you can walk away, but feel free to check on Unagi whenever you want. It’s tracking the number of exchange cycles completed, provides info on the current filtration cycle, and displays the percent exchanged for each sample. If you’re in a pinch, you can pause the run to take out samples that are done before the rest or keep it rolling. Either way, Unagi will tell you when it’s a wrap.
Take it down
Some samples are too dilute and need a little TLC. Unagi can concentrate these samples to a usable range. Choose the Reduce Sample Volume application and Unagi will concentrate up to eight dilute samples from 48 mL down to 8 mL in a single run. From there, you can follow up with a buffer exchange run or concentrate them further.
Get the check
Unagi tracks every single move it made and documents everything that happened during the run. It kicks out a detailed report of final volumes and concentrations, flow rates for every cycle, and the final percent exchanged for each sample. These reports can easily be placed in a lab notebook, uploaded to a LIMs, or hung on your refrigerator.
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Specifications
| Application | |
|---|---|
| Buffer exchange volume range | 0.5-8 mL |
| Formulations | Up to 8 formulations in parallel |
| Sample types | Antibodies and other proteins, nucleic acids, and AAVs |
| Exchange time at full volume (96%, at 10 mg/mL IgG) | 4.5 hours* |
| Protein concentration (range) | Up to 200 mg/mL* |
| Target concentration accuracy | ±10%* |
| Sample recovery | ≥96%* |
| System | |
|---|---|
| Volume measurement | Ultrasonic sensor |
| Exchange pressures | 15, 30 or 60 psi |
| Operating temperature | Room temperature |
| Buffer exchange orbital mixing | Optimized at 700 rpm Duty cycle programmable |
| Physical | 63 cm W x 57 cm D x 66 cm H; 65 kg |
| Electrical | Voltage 100-260 VAC, 50-60 Hz |
| Nitrogen or CDA requirement | Pressure 0.55–0.69 MPa (80–100 psi) Flow rate 0.25 L/s (0.5 cfm) minimum |
| Consumables | |
|---|---|
| Una | 10, 30, and 100 kDa, regenerated cellulose 30 and 100 kDa polyethersulfone |
| Disposable tips | 1000 µL non-filtered, automatic re-use up to 12 times per exchange |
| Dispense precision | 0.3-8 mL: ≤1% |
